SVI TESTOVI ZA TESTIRANJE WHO TREBAJU NAĆI POZITIVAN REZULTAT KOD SVIH LJUDI
Može li netko objasniti ovo drugačije?
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https://pieceofmindful.com/2020/04/06/bombshell-who-coronavi…
BOMBSHELL: WHO Coronavirus PCR Test Primer Sequence is Found in All Human DNA
This was important enough that I wanted to get it out immediately. My research into the NCBI database for nucleotide sequences has lead to a stunning discovery. One of the WHO primer sequences in the PCR test for SARS-CoV-2 is found in all human DNA!
The sequence “CTCCCTTTGTTGTGTTGT” is an 18-character primer sequence found in the WHO coronavirus PCR testing protocol document. The primer sequences are what get amplified by the PCR process in order to be detected and designated a “positive” test result. It just so happens this exact same 18-character sequence, verbatim, is also found on Homo sapiens chromosome 8! As far as I can tell, this means that the WHO test kits should find a positive result in all humans. Can anyone explain this otherwise?
I really cannot overstate the significance of this finding. At minimum, it should have a notable impact on test results.
Homo sapiens chromosome 8, GRCh38.p12 Primary Assembly
Sequence ID: NC_000008.11 Length: 145138636
Range 1: 63648346 to 63648363 is “CTCCCTTTGTTGTGTTGT”
Update: After some effort, I have finally discovered a way to display proof (beyond my screenshots) that human chromosome 8 has this exact same 18-character sequence. Please try the link below. The sequence is shown at the bottom of the page.
PRIJEVOD:
BOMBSHELL: Slijedi PCR testni test za coronavirus PCR, koji se nalazi u cijeloj ljudskoj DNA
Ovo je bilo dovoljno važno da sam to želio odmah objaviti. Moje istraživanje baze podataka NCBI za nukleotidne sekvence dovelo je do zapanjujućeg otkrića. Jedna od sljedova SZO početnica u PCR testu za SARS-CoV-2 nalazi se u cijeloj ljudskoj DNA!
Slijed „CTCCCTTTGTTGTGTTGT“ je sekvenca početnih slova od 18 znakova koja se nalazi u dokumentu protokola za testiranje PCR-a za koronavirus WHO-a. Slijedeće početnice pojačavaju se PCR postupkom kako bi se otkrilo i označilo kao “pozitivan” rezultat testa. Slučajno se točno taj slijed od 18 znakova, doslovno, nalazi i na Homo sapiens kromosomu 8! Koliko znam, to znači da svi setovi za testiranje SZO trebaju naći pozitivan rezultat kod svih ljudi. Može li netko objasniti ovo drugačije?
Stvarno ne mogu precijeniti značaj ovog nalaza. To bi najmanje trebalo imati značajan utjecaj na rezultate ispitivanja.
Homo sapiens kromosom 8, GRCh38.p12 Primarni sklop
ID slijeda: NC_000008.11 Duljina: 145138636
Raspon 1: 63648346 do 63648363 je “CTCCCTTTGTTGTGTTGT”
Ažuriranje: Nakon određenog napora, napokon sam otkrio način da prikažem dokaz (osim mojih snimaka zaslona) da ljudski kromosom 8 ima potpuno isti slijed od 18 znakova. Isprobajte vezu u nastavku. Slijed je prikazan na dnu stranice.
A to izgleda ovako:
Homo sapiens chromosome 8, GRCh38.p13 Primary Assembly
NCBI Reference Sequence: NC_000008.11
LOCUS NC_000008 18 bp DNA linear CON 17-AUG-2020
DEFINITION Homo sapiens chromosome 8, GRCh38.p13 Primary Assembly.
ACCESSION NC_000008 REGION: 63648346..63648363
VERSION NC_000008.11
DBLINK BioProject: PRJNA168
Assembly: GCF_000001405.39
KEYWORDS RefSeq.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 18)
AUTHORS Nusbaum,C., Mikkelsen,T.S., Zody,M.C., Asakawa,S., Taudien,S.,
Garber,M., Kodira,C.D., Schueler,M.G., Shimizu,A., Whittaker,C.A.,
Chang,J.L., Cuomo,C.A., Dewar,K., FitzGerald,M.G., Yang,X.,
Allen,N.R., Anderson,S., Asakawa,T., Blechschmidt,K., Bloom,T.,
Borowsky,M.L., Butler,J., Cook,A., Corum,B., DeArellano,K.,
DeCaprio,D., Dooley,K.T., Dorris,L. III, Engels,R., Glockner,G.,
Hafez,N., Hagopian,D.S., Hall,J.L., Ishikawa,S.K., Jaffe,D.B.,
Kamat,A., Kudoh,J., Lehmann,R., Lokitsang,T., Macdonald,P.,
Major,J.E., Matthews,C.D., Mauceli,E., Menzel,U., Mihalev,A.H.,
Minoshima,S., Murayama,Y., Naylor,J.W., Nicol,R., Nguyen,C.,
O’Leary,S.B., O’Neill,K., Parker,S.C., Polley,A., Raymond,C.K.,
Reichwald,K., Rodriguez,J., Sasaki,T., Schilhabel,M., Siddiqui,R.,
Smith,C.L., Sneddon,T.P., Talamas,J.A., Tenzin,P., Topham,K.,
Venkataraman,V., Wen,G., Yamazaki,S., Young,S.K., Zeng,Q.,
Zimmer,A.R., Rosenthal,A., Birren,B.W., Platzer,M., Shimizu,N. and
Lander,E.S.
TITLE DNA sequence and analysis of human chromosome 8
JOURNAL Nature 439 (7074), 331-335 (2006)
PUBMED 16421571
REFERENCE 2 (bases 1 to 18)
CONSRTM International Human Genome Sequencing Consortium
TITLE Finishing the euchromatic sequence of the human genome
JOURNAL Nature 431 (7011), 931-945 (2004)
PUBMED 15496913
REFERENCE 3 (bases 1 to 18)
AUTHORS Lander,E.S., Linton,L.M., Birren,B., Nusbaum,C., Zody,M.C.,
Baldwin,J., Devon,K., Dewar,K., Doyle,M., FitzHugh,W., Funke,R.,
Gage,D., Harris,K., Heaford,A., Howland,J., Kann,L., Lehoczky,J.,
LeVine,R., McEwan,P., McKernan,K., Meldrim,J., Mesirov,J.P.,
Miranda,C., Morris,W., Naylor,J., Raymond,C., Rosetti,M.,
Santos,R., Sheridan,A., Sougnez,C., Stange-Thomann,N.,
Stojanovic,N., Subramanian,A., Wyman,D., Rogers,J., Sulston,J.,
Ainscough,R., Beck,S., Bentley,D., Burton,J., Clee,C., Carter,N.,
Coulson,A., Deadman,R., Deloukas,P., Dunham,A., Dunham,I.,
Durbin,R., French,L., Grafham,D., Gregory,S., Hubbard,T.,
Humphray,S., Hunt,A., Jones,M., Lloyd,C., McMurray,A., Matthews,L.,
Mercer,S., Milne,S., Mullikin,J.C., Mungall,A., Plumb,R., Ross,M.,
Shownkeen,R., Sims,S., Waterston,R.H., Wilson,R.K., Hillier,L.W.,
McPherson,J.D., Marra,M.A., Mardis,E.R., Fulton,L.A.,
Chinwalla,A.T., Pepin,K.H., Gish,W.R., Chissoe,S.L., Wendl,M.C.,
Delehaunty,K.D., Miner,T.L., Delehaunty,A., Kramer,J.B., Cook,L.L.,
Fulton,R.S., Johnson,D.L., Minx,P.J., Clifton,S.W., Hawkins,T.,
Branscomb,E., Predki,P., Richardson,P., Wenning,S., Slezak,T.,
Doggett,N., Cheng,J.F., Olsen,A., Lucas,S., Elkin,C.,
Uberbacher,E., Frazier,M., Gibbs,R.A., Muzny,D.M., Scherer,S.E.,
Bouck,J.B., Sodergren,E.J., Worley,K.C., Rives,C.M., Gorrell,J.H.,
Metzker,M.L., Naylor,S.L., Kucherlapati,R.S., Nelson,D.L.,
Weinstock,G.M., Sakaki,Y., Fujiyama,A., Hattori,M., Yada,T.,
Toyoda,A., Itoh,T., Kawagoe,C., Watanabe,H., Totoki,Y., Taylor,T.,
Weissenbach,J., Heilig,R., Saurin,W., Artiguenave,F., Brottier,P.,
Bruls,T., Pelletier,E., Robert,C., Wincker,P., Smith,D.R.,
Doucette-Stamm,L., Rubenfield,M., Weinstock,K., Lee,H.M.,
Dubois,J., Rosenthal,A., Platzer,M., Nyakatura,G., Taudien,S.,
Rump,A., Yang,H., Yu,J., Wang,J., Huang,G., Gu,J., Hood,L.,
Rowen,L., Madan,A., Qin,S., Davis,R.W., Federspiel,N.A.,
Abola,A.P., Proctor,M.J., Myers,R.M., Schmutz,J., Dickson,M.,
Grimwood,J., Cox,D.R., Olson,M.V., Kaul,R., Raymond,C., Shimizu,N.,
Kawasaki,K., Minoshima,S., Evans,G.A., Athanasiou,M., Schultz,R.,
Roe,B.A., Chen,F., Pan,H., Ramser,J., Lehrach,H., Reinhardt,R.,
McCombie,W.R., de la Bastide,M., Dedhia,N., Blocker,H.,
Hornischer,K., Nordsiek,G., Agarwala,R., Aravind,L., Bailey,J.A.,
Bateman,A., Batzoglou,S., Birney,E., Bork,P., Brown,D.G.,
Burge,C.B., Cerutti,L., Chen,H.C., Church,D., Clamp,M.,
Copley,R.R., Doerks,T., Eddy,S.R., Eichler,E.E., Furey,T.S.,
Galagan,J., Gilbert,J.G., Harmon,C., Hayashizaki,Y., Haussler,D.,
Hermjakob,H., Hokamp,K., Jang,W., Johnson,L.S., Jones,T.A.,
Kasif,S., Kaspryzk,A., Kennedy,S., Kent,W.J., Kitts,P.,
Koonin,E.V., Korf,I., Kulp,D., Lancet,D., Lowe,T.M., McLysaght,A.,
Mikkelsen,T., Moran,J.V., Mulder,N., Pollara,V.J., Ponting,C.P.,
Schuler,G., Schultz,J., Slater,G., Smit,A.F., Stupka,E.,
Szustakowski,J., Thierry-Mieg,D., Thierry-Mieg,J., Wagner,L.,
Wallis,J., Wheeler,R., Williams,A., Wolf,Y.I., Wolfe,K.H.,
Yang,S.P., Yeh,R.F., Collins,F., Guyer,M.S., Peterson,J.,
Felsenfeld,A., Wetterstrand,K.A., Patrinos,A., Morgan,M.J., de
Jong,P., Catanese,J.J., Osoegawa,K., Shizuya,H., Choi,S. and
Chen,Y.J.
CONSRTM International Human Genome Sequencing Consortium
TITLE Initial sequencing and analysis of the human genome
JOURNAL Nature 409 (6822), 860-921 (2001)
PUBMED 11237011
REMARK Erratum:[Nature 2001 Aug 2;412(6846):565]
COMMENT REFSEQ INFORMATION: The reference sequence is identical to
On Feb 3, 2014 this sequence version replaced NC_000008.10.
Assembly Name: GRCh38.p13 Primary Assembly
The DNA sequence is composed of genomic sequence, primarily
finished clones that were sequenced as part of the Human Genome
Project. PCR products and WGS shotgun sequence have been added
where necessary to fill gaps or correct errors. All such additions
are manually curated by GRC staff. For more information see:
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Status :: Updated annotation
Annotation Name :: Homo sapiens Updated Annotation
Annotation Version :: 109.20200815
Annotation Pipeline :: NCBI eukaryotic genome annotation
pipeline
Annotation Software Version :: 8.5
Annotation Method :: Best-placed RefSeq; propagated
RefSeq model
Features Annotated :: Gene; mRNA; CDS; ncRNA
##Genome-Annotation-Data-END##
source 1..18
/organism=”Homo sapiens”
/mol_type=”genomic DNA”
/db_xref=”taxon:9606“
/chromosome=”8″
ORIGIN
PRIJEVOD:
Homo sapiens kromosom 8, GRCh38.p13 Primarni sklop
NCBI referentna sekvenca: NC_000008.11 FASTA grafika LOCUS NC_000008 linearni CON 18-kolovoz-2020, 18 bp DEFINICIJA Homo sapiens kromosom 8, GRCh38.p13 Primarni sklop. PRISTUP NC_000008 REGION: 63648346..63648363 VERZIJA NC_000008.11 DBLINK BioProject: PRJNA168 Montaža: GCF_000001405.39 KLJUČNE RIJEČI RefSeq. IZVOR Homo sapiens (čovjek) ORGANIZAM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; vertebrata; Euteleostomi; Sisari; Eutheria; Euarchontoglires; Primati; haplorrhini; catarrhini; veliki čovjekoliki majmuni; Homo.
LITERATURA 1 (osnove 1 do 18)
AUTORI:
Nusbaum,C., Mikkelsen,T.S., Zody,M.C., Asakawa,S., Taudien,S.,
Garber,M., Kodira,C.D., Schueler,M.G., Shimizu,A., Whittaker,C.A.,
Chang,J.L., Cuomo,C.A., Dewar,K., FitzGerald,M.G., Yang,X.,
Allen,N.R., Anderson,S., Asakawa,T., Blechschmidt,K., Bloom,T.,
Borowsky,M.L., Butler,J., Cook,A., Corum,B., DeArellano,K.,
DeCaprio,D., Dooley,K.T., Dorris,L. III, Engels,R., Glockner,G.,
Hafez,N., Hagopian,D.S., Hall,J.L., Ishikawa,S.K., Jaffe,D.B.,
Kamat,A., Kudoh,J., Lehmann,R., Lokitsang,T., Macdonald,P.,
Major,J.E., Matthews,C.D., Mauceli,E., Menzel,U., Mihalev,A.H.,
Minoshima,S., Murayama,Y., Naylor,J.W., Nicol,R., Nguyen,C.,
O’Leary,S.B., O’Neill,K., Parker,S.C., Polley,A., Raymond,C.K.,
Reichwald,K., Rodriguez,J., Sasaki,T., Schilhabel,M., Siddiqui,R.,
Smith,C.L., Sneddon,T.P., Talamas,J.A., Tenzin,P., Topham,K.,
Venkataraman,V., Wen,G., Yamazaki,S., Young,S.K., Zeng,Q.,
Zimmer,A.R., Rosenthal,A., Birren,B.W., Platzer,M., Shimizu,N. and
Lander,E.S.
NASLOV Slijed DNA i analiza humanog kromosoma 8
ČASOPIS Nature 439 (7074), 331-335 (2006)
DOSTAVLJENO 16421571
REFERENCA 2 (osnove 1 do 18)
CONSRTM Međunarodni konzorcij za sekvenciranje ljudskog genoma
NASLOV Završavanje euhromatskog slijeda ljudskog genoma
ČASOPIS Nature 431 (7011), 931-945 (2004)
DOSTAVLJENO 15496913
REFERENCA 3 (osnove 1 do 18)
AUTORI:
Lander,E.S., Linton,L.M., Birren,B., Nusbaum,C., Zody,M.C.,
Baldwin,J., Devon,K., Dewar,K., Doyle,M., FitzHugh,W., Funke,R.,
Gage,D., Harris,K., Heaford,A., Howland,J., Kann,L., Lehoczky,J.,
LeVine,R., McEwan,P., McKernan,K., Meldrim,J., Mesirov,J.P.,
Miranda,C., Morris,W., Naylor,J., Raymond,C., Rosetti,M.,
Santos,R., Sheridan,A., Sougnez,C., Stange-Thomann,N.,
Stojanovic,N., Subramanian,A., Wyman,D., Rogers,J., Sulston,J.,
Ainscough,R., Beck,S., Bentley,D., Burton,J., Clee,C., Carter,N.,
Coulson,A., Deadman,R., Deloukas,P., Dunham,A., Dunham,I.,
Durbin,R., French,L., Grafham,D., Gregory,S., Hubbard,T.,
Humphray,S., Hunt,A., Jones,M., Lloyd,C., McMurray,A., Matthews,L.,
Mercer,S., Milne,S., Mullikin,J.C., Mungall,A., Plumb,R., Ross,M.,
Shownkeen,R., Sims,S., Waterston,R.H., Wilson,R.K., Hillier,L.W.,
McPherson,J.D., Marra,M.A., Mardis,E.R., Fulton,L.A.,
Chinwalla,A.T., Pepin,K.H., Gish,W.R., Chissoe,S.L., Wendl,M.C.,
Delehaunty,K.D., Miner,T.L., Delehaunty,A., Kramer,J.B., Cook,L.L.,
Fulton,R.S., Johnson,D.L., Minx,P.J., Clifton,S.W., Hawkins,T.,
Branscomb,E., Predki,P., Richardson,P., Wenning,S., Slezak,T.,
Doggett,N., Cheng,J.F., Olsen,A., Lucas,S., Elkin,C.,
Uberbacher,E., Frazier,M., Gibbs,R.A., Muzny,D.M., Scherer,S.E.,
Bouck,J.B., Sodergren,E.J., Worley,K.C., Rives,C.M., Gorrell,J.H.,
Metzker,M.L., Naylor,S.L., Kucherlapati,R.S., Nelson,D.L.,
Weinstock,G.M., Sakaki,Y., Fujiyama,A., Hattori,M., Yada,T.,
Toyoda,A., Itoh,T., Kawagoe,C., Watanabe,H., Totoki,Y., Taylor,T.,
Weissenbach,J., Heilig,R., Saurin,W., Artiguenave,F., Brottier,P.,
Bruls,T., Pelletier,E., Robert,C., Wincker,P., Smith,D.R.,
Doucette-Stamm,L., Rubenfield,M., Weinstock,K., Lee,H.M.,
Dubois,J., Rosenthal,A., Platzer,M., Nyakatura,G., Taudien,S.,
Rump,A., Yang,H., Yu,J., Wang,J., Huang,G., Gu,J., Hood,L.,
Rowen,L., Madan,A., Qin,S., Davis,R.W., Federspiel,N.A.,
Abola,A.P., Proctor,M.J., Myers,R.M., Schmutz,J., Dickson,M.,
Grimwood,J., Cox,D.R., Olson,M.V., Kaul,R., Raymond,C., Shimizu,N.,
Kawasaki,K., Minoshima,S., Evans,G.A., Athanasiou,M., Schultz,R.,
Roe,B.A., Chen,F., Pan,H., Ramser,J., Lehrach,H., Reinhardt,R.,
McCombie,W.R., de la Bastide,M., Dedhia,N., Blocker,H.,
Hornischer,K., Nordsiek,G., Agarwala,R., Aravind,L., Bailey,J.A.,
Bateman,A., Batzoglou,S., Birney,E., Bork,P., Brown,D.G.,
Burge,C.B., Cerutti,L., Chen,H.C., Church,D., Clamp,M.,
Copley,R.R., Doerks,T., Eddy,S.R., Eichler,E.E., Furey,T.S.,
Galagan,J., Gilbert,J.G., Harmon,C., Hayashizaki,Y., Haussler,D.,
Hermjakob,H., Hokamp,K., Jang,W., Johnson,L.S., Jones,T.A.,
Kasif,S., Kaspryzk,A., Kennedy,S., Kent,W.J., Kitts,P.,
Koonin,E.V., Korf,I., Kulp,D., Lancet,D., Lowe,T.M., McLysaght,A.,
Mikkelsen,T., Moran,J.V., Mulder,N., Pollara,V.J., Ponting,C.P.,
Schuler,G., Schultz,J., Slater,G., Smit,A.F., Stupka,E.,
Szustakowski,J., Thierry-Mieg,D., Thierry-Mieg,J., Wagner,L.,
Wallis,J., Wheeler,R., Williams,A., Wolf,Y.I., Wolfe,K.H.,
Yang,S.P., Yeh,R.F., Collins,F., Guyer,M.S., Peterson,J.,
Felsenfeld,A., Wetterstrand,K.A., Patrinos,A., Morgan,M.J., de
Jong,P., Catanese,J.J., Osoegawa,K., Shizuya,H., Choi,S. and
Chen,Y.J.
CONSRTM Međunarodni konzorcij za sekvenciranje ljudskog genoma
NASLOV Početno sekvenciranje i analiza ljudskog genoma
ČASOPIS Nature 409 (6822), 860-921 (2001)
DOSTAVLJENO 11237011
NAPOMENA Erratum: [Nature 2001, 2. kolovoza; 412 (6846): 565]
INFORMACIJE ZA REFSEQ KOMENTAR: Referentni niz identičan je
CM000670.2.
Dana 3. veljače 2014. ova je sekvencijalna verzija zamijenila NC_000008.10.
Naziv sklopa: GRCh38.p13 Primarni sklop
DNA sekvenca sastoji se prvenstveno od genomske sekvence
gotovi klonovi koji su sekvencionirani kao dio ljudskog genoma
Projekt. Dodani su PCR proizvodi i WGS sekvenca sačmarica
po potrebi popuniti praznine ili ispraviti pogreške. Svi takvi dodaci
su ručno kurirani od strane GRC osoblja. Za više informacija pogledajte:
https://genomereference.org.
##-Genome Primjedba-podataka START ##
Pružatelj bilješki :: NCBI
Status bilješke :: Ažurirana bilješka
Naziv bilješke :: Homo sapiens Ažurirana bilješka
Izdanje 109,20200815
Verzija napomene :: 109.20200815
Cjevovod za bilješke: Oznaka eukariotskog genoma NCBI cjevovod
Verzija softvera za napomene: 8.5
Metoda bilježenja :: Najbolje postavljeni RefSeq; Razmnožava RefSeq model
Značajke s bilješkama: Gene; mRNA; CDS; ncRNA
##-Genome Primjedba-podataka END ##
OSOBINE Mjesto / Kvalifikacije
izvor 1..18
/ organizam = "Homo sapiens"
/ mol_type = "genomska DNK"
/ Db_xref = "svojta: 9606"
/ Kromosom = "8"
PODRIJETLO
1 ctccctttgt tgtgttgt
Pripremila: mr. sc. Arna Šebalj
